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Recombinant proteins are widely used in biological and biomedical research[1], and recombinant protein expression has become a commonly used tool. Nevertheless, there are still many aspects to consider before starting an expression project. For example, which expression system is chosen for obtaining the protein of interest? Should it be expressed in bacteria, in yeast, in insect cells or in mammalian cells? What is the best expression vector to be used? As to the bacterial expression system, which strain should be chosen? Is there any differences in the expression condition between the full-
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Molecular dynamics (MD) simulation is a computer simulation technique that allows one to predict the time dependent evolution of a system of interacting particles using laws of physics. Studying the dynamic development of a biological system with the consideration of protein flexibility is of vital importance, since many biological phenomena involving proteins are very dynamic processes, which include transport, molecular recognition, enzyme catalysis, and allosteric regulation. The static models produced by NMR, X-ray crystallography, and homology modeling offer valuable insights into macrom
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Recombinant protein expression is the technique of target protein production using the recombinant DNA technology. It is now commonly used in bioresearch and in pharmaceutical production of recombinant protein therapeutics. The recombinant DNA, usually the cDNA sequence of the target protein, is designed to be under the control of a well characterized promoter and express the target protein within the chosen host cell to achieve high-level protein expression.